Powerful PowerPoint - Activators Patch

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Powerful PowerPoint - Activators Patch

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PDF Password Recovery – Recover lost passwords for protected PDF files (* .pdf) with GPU acceleration. Instantly remove PDF restrictions for editing, copying, printing, and extracting data.To establish an improved, high-throughput screening techniques for identifying novel KCNQ2 channel activators. KCNQ2 channels were stably expressed in CHO cells (KCNQ2 cells). Thallium flux assay was used for primary screening, and 384-well automated patch-clamp Ion Works Barracuda was used for hit validation. Two validated activators were characterized using a conventional patch-clamp recording technique. From a collection of 80 000 compounds, the primary screening revealed a total of 565 compounds that potentiated the fluorescence signals in thallium flux assay by more than 150%. When the 565 hits were examined in Ion Works Barracuda, 38 compounds significantly enhanced the outward currents recorded in KCNQ2 cells, and were confirmed as KCNQ2 activators. In the conventional patch-clamp recordings, two validated activators ZG1732 and ZG2083 enhanced KCNQ2 currents with EC50 values of 1.04±0.18 μmol/L and 1.37±0.06 μmol/L, respectively. The combination of thallium flux assay and Ion Works Barracuda assay is an efficient high-throughput screening (HTS) route for discovering KCNQ2 activators. With more than 40 members, voltage-gated potassium (Kv) channels are a major drug target group that is essential for treating a variety of human diseases. The neuronal KCNQ2 potassium channel (Kv7.2) is a low-threshold Kv channel that is expressed in both the central and peripheral nervous systems. As an essential player that mediates neuronal muscarinic (M) currents and controls neuronal excitability, activating a KCNQ2 channel or heterogeneous KCNQ2 complexes will inhibit action potential initiation and, thus, dampen neuronal excitability. Activation of the KCNQ2 channel by synthetic compounds may dampen membrane excitability and, therefore, may be a strategy for treating hyperexcitability conditions, such as epilepsy and neuropathic pain. Identifying novel activators is beneficial for understanding channel activity mechanisms and developing new therapeutics. For example, one study used ztz240 as a chemical probe to demonstrate that the KCNQ2 channel voltage-sensing domains are novel anti-epilepsy targets. Benzbromarone (BBR) is a KCNQ activator that primarily acts upon peripheral KCNQ channels. The unique tissue distribution of BBR allowed scientists to resolve a long-standing debate concerning the central or peripheral mediation of analgesic activity of KCNQ activators. This provided a new strategy for developing more effective pain therapies. To discover novel small molecule activators for KCNQ2 channels from large compound libraries, various high-throughput screening (HTS) techniques have been developed. Fluorescence-based assays provide robust and homogeneous cell population measurements and have been widely used for both cation and anion-permeable channels. Electrophysiological recording directly provides ionic currents and is considered to be a gold standard for studying ion channels. Nevertheless, due to their low throughput, conventional patch-clamp experiments are not appropriate for large-scale screening. Over the past few decades, many automated electrophysiology platforms with highly improved throughput have been developed. Therefore, directly measuring ionic currents in HTS is a reality. The Ion Works Barracuda is a 384-well format automated patch that relies on a perforated patch-clamp technique. Compared with earlier generations of this instrument, the Ion Works Barracuda provides better throughput and data consistency; however, the feasibility of using the Ion Works Barracuda in a KCNQ2 study has not been reported. Herein, we screened a collection of 80 000 compounds using the thallium flux assay as the primary screen and then used the Ion Works Barracuda for a secondary validation. Two validated hits were further characterized using a conventional patch clamp, which revealed an ECFlow-chart of the high-throughput screening route for identifying KCNQ2 activators. The fluorescence-based thallium flux assay was used for the primary screen, followed by the confirmatory 384-well Ion Works Barracuda for hit validation. After the hit validation, a conventional patch-clamp experiment was used for probe characterization. Power Point slide Chinese hamster ovary (CHO) cells that stably expressed KCNQ2 channels were routinely cultured in F-12 medium supplemented with 10% FBS and 250 μg/m L Hygromycin B. KCNQ2 cells were seeded into a 384-well black clear bottom plate at a concentration of 8000 cells/well by a multidrop dispenser, and the plate was incubated overnight at 37 °C in a 5% CO incubator. The next day, after manual removal of the medium, 25 μL/well dye-loading buffer (1000× Flux OR reagent stock, 100× Power Load, 1× Hanks' balanced salt solution, 20 mmol/L HEPES, and 2.5 mmol/L Probenecid, p H 7.40) was added into the cell plate, and the plate was then incubated in the dark at room temperature for 90 min. Once the dye-loading buffer was manually removed, the cell plates were then supplemented with 20 μL/well assay buffer (1× Hank's balanced salt solution, 20 mmol/L HEPES, and 2.5 mmol/L Probenecid, p H 7.40). Additionally, 4 μL/well compound buffer was added into the plate, and the plate was then incubated at room temperature in the dark. After the tested compounds were incubated for 30 min, cell plates were loaded onto an FDSS (Hamamatsu Photonics). After 10 s of recording, 6 μL/well of stimulus buffer was added. The stimulus buffer contained 25 mmol/L K are the standard deviation of the control group (c) and the group in the presence of positive control group (n), respectively. C and N are the mean values of the control and positive control groups, respectively. KCNQ2 cells were dissociated from T175 flasks by aspirating off the culture medium, rinsing the cells twice with phosphate buffered saline solution (PBS, Invitrogen) and adding 3 m L trypsin-EDTA per flask. The cells were incubated for 3 min in a 37 °C incubator. The flasks were added to 7 m L of cell culture medium, and the cells were gently triturated by pipetting up and down two to three times. Cells were pelleted at 800 rounds per minute for 3 min. The supernatant was discarded and the cell pellet was resuspended in 5 m L of external solution at a density of 1.5–2.0 million cells per m L. The 384-well automated patch clamp Ion Works Barracuda (Molecular Devices) in the population patch clamp (PPC) mode was used for hit validation. The cells were voltage clamped at a holding potential of −100 m V. The KCNQ2 current was activated by depolarizing to −10 m V for 10 s, after which the voltage was taken back to −120 m V for 10 s to observe the deactivating tail current. The maximum amount of outward current size was used to determine KCNQ2 current amplitude. Standard whole-cell recording was used to record the current of KCNQ2 channels. Pipettes were pulled from borosilicate glass capillaries (TW150-4, World Precision Instruments, Sarasota, FL, USA). When filled with the intracellular solution, the pipettes had a 3–5 MΩ resistance. During the recording, the extracellular solution was constantly perfused by a BPS perfusion system (ALA Scientific Instruments). The intracellular solution contained (in mmol/L): 140 KCl, 1.75 Mg Cl, 10 HEPES and 10 glucose (p H=7.40). Both currents and voltages were recorded using an Axopatch-200B amplifier, filtered at 2 k Hz, and digitized using a Digi Data 1440A with p Clamp 10.2 software (Molecular Devices). Series resistance compensation was also used and set to 60%–80%. Patch clamp data were processed using Clampfit 10.2 (Molecular Devices, Sunnyvale, CA, USA) and then analyzed in Graph Pad Prism 5 (Graph Pad Software). Voltage-dependent activation curves were fitted using the Boltzmann equation, G=G is the drug concentration that produced half of the maximum response, and P is the Hill coefficient. The significance was estimated using paired two-tailed Student's t-tests. An effect was considered significant if PTo identify compounds with potentiation effects on KCNQ2 channels, we generated a KCNQ2-stable cell line. After a series of optimization experiments for several important screening parameters, which have been described previously, a standard procedure for the thallium flux assay was developed and used to screen an in-house collection of over 80 000 compounds at a 10 μmol/L final concentration (Figure 2). The positive control was ztz240, which is a KCNQ2 channel activator that was previously identified to significantly increase the fluorescence signal by approximately 150% at 10 μmol/L. Of the 80 000 screened compounds, 565 hits were discovered that exhibited a larger potentiation activity than ztz240, as exemplified by ZG1732. The Z factors ranged from 0.51 to 0.89, which are consistent with the assay quality requirement of being greater than 0.5. (A and B) Representative fluorescence traces in the absence or presence of 10 μmol/L ztz240 (A) or 10 μmol/L ZG1732 (B). (D) Distribution of identified hits in a total of 80 000 compounds in the thallium flux assay. The x-axis represents the potentiation activity of the hits on the KCNQ2-mediated fluorescence signal (see the Methods for the calculation method). Power Point slide A 384-well automated patch-clamp instrument, the Ion Works Barracuda, was used to validate the hits. The stabilities of the KCNQ2 currents were evaluated first. The cells were held at −100 m V and stimulated by depolarization to −10 m V every 20 s. Prior to each depolarization event, a 20-ms pre-pulse that polarizes the membrane potential to −90 m V (a “sweep”) was delivered for leak subtraction. Under these conditions, the outward currents of the KCNQ2 channels remained relatively stable for 5 min. We then examined the KCNQ2 current responses to 10 μmol/L ztz240. As shown in Figure 3, the drug was applied after the 3rd sweep. The increased KCNQ2 currents reached a steady state by the 5th sweep, and a slight run-down was observed at the 7th sweep. Therefore, the currents measured at the 6th sweep were used to determine the potentiation activity of the tested hits. Of the 565 hits that were discovered in the primary screen, 38 were confirmed as KCNQ2 channel activators (Figure 3C and 3D, and Supplementary Table S1). Most of the validated activators displayed comparable potentiation activity to ztz240, which is exemplified by ZG1732. (A) Stability of KCNQ2 currents that were recorded using the Ion Works Barracuda instrument. ztz240 (10 μmol/L) was applied at the 3rd sweep (a). The increased KCNQ2 current was measured before the 'run-down' appeared (the 6th sweep (b). (B) Representative KCNQ2 currents before and after exposure to 10 μmol/L ztz240. (C) Potentiation of the validated hits on the KCNQ2 currents. (D) Representative KCNQ2 currents before and after exposure to 10 μmol/L ZG1732 [red point in (C)]. Power Point slide Among the 38 validated activators, ZG1732 and ZG2083, which belong to different scaffolds, were selected for further characterization using a conventional whole-cell patch-clamp, the gold standard for studying ion channels. Similar to the KCNQ2 activators that were identified previously, including retigabine and ztz240, both ZG1732 and ZG2083 increased the outward KCNQ2 currents and shifted the activation curve to the left (Figure 4). The ECPotentiation effects of the hits ZG1732 and ZG2083 on KCNQ2 channels using a conventional patch-clamp. (A) Representative currents of KCNQ2 channels in the absence or presence of indicated hits. To elicit the KCNQ2 currents, depolarization steps that ranged from −90 m V to 60 m V in 10 m V increments were applied from the holding potential of −100 m V. (B and C) ZG1732 and ZG2083 left-shifted the conductance-voltage (G-V) curve of KCNQ2 channels. For ZG1732, ΔVIn the current study, the thallium flux assay was used as the primary screening method, and the 384-well Ion Works Barracuda instrument was used for the secondary validation. Currently, the thallium flux assay remains the prevalent HTS method for identifying potassium channel modulators, including KCNQ channels. The thallium assay can be used to identify both activators and inhibitors of KCNQ channels. Typically, the hits identified in a primary screen require further chemical modification to improve potency and druggability. For example, a potent KCNQ1 activator was developed based upon an initial hit that was identified in an HTS of the Molecular Libraries Probe Centers Network (MLPCN) library using the thallium assay; however, due to the off-target effects and autofluorescence of the compounds, a high false positive hit rate is a major issue for this indirect fluorescence assay. In a screen for KCNQ4 activators, the false positive rate was greater than 93% (623/666) in the primary screening stage. Consistent with this previous study, the false positive rate for our thallium assay was also 93% (527/565). To reduce the false positive rate, a secondary confirmation is often required with duplicates for each compound and counter screening for the parental cells that do not express the KCNQ channels investigated. A conventional patch-clamp experiment is the gold standard for studying ion channels and provides a large quantity of information as well as high resolution; however, due to intrinsic limitations, particularly due to its low throughput, conventional patch-clamp is not able to satisfy the need of high-throughput drug discovery. These automated patch-clamp systems can be divided into automated conventional electrophysiology platforms that use glass pipettes and automated planar-array electrophysiology platforms. Nevertheless, only the latter have overcome the throughput limitations and were first used for screening in 2003. The Ion Works Barracuda is a 384-well planar-array platform that can simultaneously record 384 currents. As a high-throughput patch-clamp instrument that is specifically designed for high-throughput drug discovery, the utility of Ion Works Barracuda must be tested and validated for various ion channels. In the current study, the Ion Works Barracuda can stably record KCNQ2 currents for up to 5 min; thus, it is feasible to evaluate KCNQ2 activators that typically reach steady potentiation within a few minutes. The validated activators were further characterized using conventional patch clamp studies, which confirmed the Ion Works Barracuda results. In summary, our study established an efficient HTS means for identifying KCNQ2 activators, which can be referred to or is applicable for other voltage-gated potassium channels. Zhao-bing GAO and Fa-Jun NAN designed the project; Jin-feng YUE, Guan-hua QIAO, Ni Liu, and Xue-qin CHEN performed the experiments; all authors analyzed the data; Jin-feng YUE and Zhao-bing GAO wrote the manuscript. Novel KCNQ2 channel activators discovered using fluorescence-based and automated patch-clamp-based high-throughput screening techniques. We gratefully acknowledge the financial support from the National Natural Science Foundation of China Grant for Excellent Key Laboratory (81123004), the National Natural Science Foundation of China (61327014, 6117513122), Shanghai Municipal Science and Technology Commission (13JC1406700) and the External Cooperation Program of BIC, Chinese Academy of Sciences (1536631KYSB20130003). Different types of Scaffolding used for various types of construction. 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